Project

Part:BBa_K1419005:Design

Designed by: Harland Brandon   Group: iGEM14_Lethbridge   (2014-10-09)

RVG-Lamp2B-Clover


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 153


Design Notes

Source

Lamp2B sequence comes from mouse genomic sequence.

The Clover protein used was from a study by Lam and colleagues (2012)

Lam, A. J., St-pierre, F., Gong, Y., Marshall, J. D., Cranfill, P. J., Baird, M. A., … Lin, M. Z. (2012). Improving FRET dynamic range with bright green and red fluorescent proteins, 9(10). doi:10.1038/NMETH.2171


References